This study is concerned with the preparation, performance testing and application of aqueous functional inks in packaging printing. The preparation process of aqueous functional inks is firstly introduced, focusing on the rheological and color properties. A comprehensive understanding of the fluidity and deformation behavior of the ink in printing is provided, which provides important process parameters for practical application. Attention is also paid to the color reproduction and stability of aqueous functional inks to ensure that the printed products have excellent visual effects in the packaging field. Finally, the wide application of aqueous functional inks in packaging printing is outlined. The correlation coefficients of red, green and blue are 0.961, 0.985 and 0.950. It provides a scientific basis for the optimization of the performance of aqueous functional inks, and at the same time provides an environmentally friendly and efficient choice for the packaging printing industry.