化学
荧光
髓系白血病
体内
荧光寿命成像显微镜
点击化学
体外
部分
吲哚试验
细胞
细胞生物学
药物发现
生物化学
白血病
生物物理学
计算生物学
癌症研究
组合化学
立体化学
物理
生物
医学
内科学
生物技术
量子力学
作者
Yong Li,Jiangong Zhang,Xiaolong Ni,Xu Wang,Jian Zhang,Xilei Xie,Xueyu Dou,Xiaoyun Jiao,Bo Tang
标识
DOI:10.1021/acs.analchem.3c04024
摘要
The antiapoptotic protein myeloid cell leukemia 1 (Mcl-1) has been increasingly identified as a promising potential therapeutic target attributed to its critical regulation effect in diverse cellar physiopathological events. Current fluorescence imaging strategies tend to be susceptible to the cellular microenvironment, and straightforward mapping of Mcl-1's level variation remains challenging. In this paper, an activatable "off–on" fluorescence strategy for Mcl-1 specific labeling was presented based on bio-orthogonal chemistry by introducing tetrazine-functionalized borondipyrromethene (TB) as a fluorescent reporter and trans-cyclooctyne-derived indole-2-carboxylic acid (TI) as an Mcl-1 targeting moiety. With the click pair of TB and TI, the Mcl-1 expression level in vitro and in vivo was successfully mapped straightforward. Also, the level changes of Mcl-1 upon drug challenge were demonstrated. This work provides a robust fluorescence strategy for Mcl-1 in situ imaging, and the results would further facilitate the comprehensive revelation of the Mcl-1 biological effect.
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