Peptide Synthesis

Peptide synthesis is characterized by forming a substituted amide linkage between two amino acids. The first successful attempt to synthetically link amino acids through peptide bonds is more than a hundred years old. However, the process and chemistry have undergone a series of optimizations, and peptide synthesis has become a more practical part of present-day high-throughput scientific research following the advent of solid-phase synthesis strategies. This article describes a generalized protocol for solid-phase peptide synthesis (SPPS) using Fmoc chemistry, including their purification and characterization by reversed-phase high-performance liquid chromatography and mass spectrometry.