上睑下垂
程序性细胞死亡
流式细胞术
细胞生物学
自噬
细胞凋亡
细胞
生物
计算生物学
免疫学
遗传学
作者
Siyuan Huang,Ling Zeng
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 165-178
被引量:1
标识
DOI:10.1007/978-1-0716-3433-2_15
摘要
Cell death is a crucial and fundamental process in the biology of all living organisms and plays an essential role in developmental, cellular, and molecular biology. With a deeper understanding of the mechanisms of different types of cell death, quantitative experiments are becoming increasingly necessary to analyze the dynamic changes and coordinate physiological processes. Flow cytometry is the most widely used method for detecting and quantifying cell processes in mammalian cells, providing a comprehensive and high-throughput approach, even at the single-cell level. This chapter provides a brief overview of guidelines for performing flow cytometry in the detection of regulated cell deaths, including apoptosis, autophagy, ferroptosis, pyroptosis, and immunogenic cell death.
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