Wnt信号通路
干细胞
细胞生物学
类有机物
生物
转录组
细胞外基质
细胞分化
信号转导
基因表达
遗传学
基因
作者
Abdulkhaliq Alsaadi,Mara Artibani,Zhiyuan Hu,Nina Wietek,Matteo Morotti,Laura Santana González,M Alazzam,Jason Zheng Jiang,Beena Abdul,Hooman Soleymani Majd,Levi L. Blazer,Arun P. Wiita,Francesca Silvestri,Sachdev S. Sidhu,Joan S. Brugge,Ahmed A. Ahmed
出处
期刊:Cell Reports
[Elsevier]
日期:2023-11-01
卷期号:42 (11): 113354-113354
标识
DOI:10.1016/j.celrep.2023.113354
摘要
The study of fallopian tube (FT) function in health and disease has been hampered by limited knowledge of FT stem cells and lack of in vitro models of stem cell renewal and differentiation. Using optimized organoid culture conditions to address these limitations, we find that FT stem cell renewal is highly dependent on WNT/β-catenin signaling and engineer endogenous WNT/β-catenin signaling reporter organoids to biomark, isolate, and characterize these cells. Using functional approaches, as well as bulk and single-cell transcriptomics analyses, we show that an endogenous hormonally regulated WNT7A-FZD5 signaling axis is critical for stem cell renewal and that WNT/β-catenin pathway-activated cells form a distinct transcriptomic cluster of FT cells enriched in extracellular matrix (ECM) remodeling and integrin signaling pathways. Overall, we provide a deep characterization of FT stem cells and their molecular requirements for self-renewal, paving the way for mechanistic work investigating the role of stem cells in FT health and disease.
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