毛孢子
医学
传统医学
微生物学
食品科学
生物
生物化学
酵母
作者
Weizheng Zhang,Yinglun Xiao,Guangyuan Deng,Xiangsheng Cai
出处
期刊:Jundishapur Journal of Microbiology
[Kowsar Medical Institute]
日期:2024-09-22
卷期号:17 (7)
摘要
Background: Trichosporon asahii is a significant yeast-like fungal species found in the environment and capable of colonizing certain human organs. The glucuronoxylomannan (GXM) of T. asahii may play a crucial role in its ability to evade the host's immune response. Objectives: To evaluate the immunomodulatory effects of T. asahii GXM, this study investigated its impact on macrophage cell viability and adenosine triphosphate (ATP) production. Methods: Glucuronoxylomannan was extracted using ethanol and cetyltrimethylammonium bromide precipitation and further purified by the CIA-modified purification method. The CCK8 kit was used to assess the effect of GXM on macrophage viability, while an ATP detection kit measured changes in ATP production in macrophages under GXM intervention. Results: The GXM of T. asahii was successfully purified using the CIA-modified method. Following GXM intervention, macrophage viability initially increased but then decreased over time. glucuronoxylomannan also significantly reduced ATP production in monocyte-derived macrophages. Conclusions: Trichosporon asahii can influence macrophages by inducing GXM-mediated alterations in energy metabolism.
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