蘑菇
碳酸钙-2
化学
细胞外
表征(材料科学)
纳米技术
生物化学
食品科学
材料科学
体外
作者
Xueli Yang,Zihao Zong,Ben Niu,Hangjun Chen,Weijie Wu,Xiangjun Fang,Ruiling Liu,Haiyan Gao,Honglei Mu
标识
DOI:10.1016/j.foodchem.2024.141339
摘要
In this study, Shiitake mushroom-derived extracellular nanovesicles (SMDENVs) were isolated from fresh Shiitake mushrooms by ultracentrifugation and sucrose gradient ultracentrifugation. The morphological characteristics of SMDENVs were investigated via Transmission Electron Microscopy and Laser Scanning Confocal Microscopy. SMDENVs were spherical, hollow, and uniform in size, with an average diameter of 177.6 ± 51.4 nm. Based on the analysis of lipidomics and proteomics, 383 lipids species and 1290 proteins were identified in SMDENVs. Compared with the conventional liposomes, SMDENVs demonstrated higher stability in different environmental conditions. Furthermore, we observed that SMDENVs were cytocompatible and inhibited the proliferation of Caco-2 cells. SMDENVs could be phagocytized by Caco-2 cells in a time-dependent manner. Further, SMDENVs also inhibited the proliferation of Caco-2 cells in a dose-dependent manner, and the half-maximal inhibitory concentration (IC
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