Anti-Microbial Activity of Aspergillus terreus Bioactive Compounds Extract

Abstract Microorganisms that possess antimicrobial properties opened up new possibilities in the fight against microbes, A very effective microorganism in combating pathogenic microbes is the Aspergillus terreus . All species of Aspergillus do not have appropriate and similar antimicrobial effects, and it is important to identify and isolate species with antimicrobial effects. In this research, moldy citrus fruits were sampled and Aspergillus was isolated and identified from them. 3 species of Aspergillus terreus , Aspergillus niger isolate 1 and Aspergillus niger isolate 2 were isolated. After that, the crude metabolites of these molds were prepared in the liquid culture medium of Sabro dextrose broth in the shaker incubator. Finally, the effect of these metabolites on Staph. saprophyta, Staph. aureus, Strep. Pyrogen, E. coli , and Candida sp. were done on this bacterium through well diffusion by determining the diameter of the growth inhibition halo of each of these metabolites, and then MIC and MBC were calculated. Findings: The average MIC of Aspergillus terreus metabolite on Staphylococcus aureus was calculated to be 208.33 μl/ml and its average MBC on this bacterium was 416.66 μl/ml. The average MIC of Aspergillus niger metabolite on Staphylococcus aureus was calculated to be 166.66 μl/ml and its average MBC was determined to be 333.33 μl/ml. with 150, 165, and 180 microliters of Aspergillus niger metabolite, the average diameter of growth inhibition halo was 8, 8, and 8.33 mm, respectively. Aspergillus produces different effects in preventing the growth of microorganisms. Also used was GC-mass analysis to detect some of the bioactive compounds in ethanol excerpts and primary statements that used four excerpts of A. terreus . The most important active composites were detected in 20 different compounds with antimicrobial and antifungal conditioning. Bioactive composites such as Hexadecanoic acid ethyl ester, Heptadecanoic acid, (E) Octadec-9-enoic acid ethyl, Tetradecanoic acid, Pentadecanoic acid, and Ethyl 9- hexadecenoic.