Antibacterial mechanism of forsythoside A against Pseudomonas syringae pv. actinidiae

丁香假单胞菌 微生物学 最低杀菌浓度 生物 病菌 管家基因 细菌 最小抑制浓度 毒力 生物膜 致病菌 柑橘天堂 基因 基因表达 抗菌剂 植物 生物化学 遗传学 芸香科
作者
Hurong Pei,Mingxiu Lu,Long Li,Zhangfu Long
出处
期刊:Microbial Pathogenesis [Elsevier]
卷期号:173: 105858-105858 被引量:6
标识
DOI:10.1016/j.micpath.2022.105858
摘要

In this study, we investigated the antibacterial mechanism of forsythoside A against the kiwifruit canker pathogen, which provided the theoretical basis for the prevention and control of canker disease and the development of plant-based fungicides. The pathogenic bacteria were isolated from kiwifruit diseased tissues and the specific primers Psa_A1 F2 and Psa_A1 R1 were used for preliminary identification. Four pairs of housekeeping genes, including gapA, gltA, gyrB, and rpoD, were used for polygenic typing identification. The inhibition effect of forsythoside A on Psa was evaluated by the filter paper bacteriostasis method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Psa were determined by the 96-well plate absorbance and colony counts. The changes in Psa biofilm formation, motility, IAA synthesis, iron utilization, and respiratory chain dehydrogenase activity were determined. The Psa morphology was observed by Scanning electron microscope (SEM) and transmission electron microscope (TEM). The expression of some virulence genes was analyzed by qPCR. The results showed that the pathogen was Pseudomonas syringae pv. actinidiae(Psa). The inhibitory effect of forsythoside A on Psa was positively correlated with its concentration. while the MIC and MBC were 2.0 and 5.0 mg/mL, respectively. The biofilm formation and motility of Psa were not only obviously inhibited, but also the substance and energy metabolism were interfered, while obvious deformity and rupture of the cells were occurred in Psa Bacteria. In addition, The transcription of the Psa pathogenic genes was affected. The infection investigation of kiwifruit leaves indicated that forsythiaside A inhibits Psa pathogenicity and had a protective effect. This study concluded that forsythoside A has a certain control effect on kiwifruit canker, and has the potentiality to be developed as a novel plant fungicide.
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