体内
生物发光
NAD+激酶
烟酰胺腺嘌呤二核苷酸
生物发光成像
生物
转移
生物化学
体外
保健品
限制
新陈代谢
翻译(生物学)
癌细胞
乳腺癌
癌症
癌症研究
荧光素酶
转染
信使核糖核酸
酶
基因
工程类
机械工程
遗传学
作者
Tamara Maric,Arkadiy Bazhin,Pavlo Khodakivskyi,Georgy Mikhaylov,Ekaterina Solodnikova,Aleksey Yevtodiyenko,Greta Maria Paola Giordano Attianese,George Coukos,Melita Irving,Magali Joffraud,Carles Cantó,Elena A. Goun
标识
DOI:10.1016/j.bios.2022.114826
摘要
Nicotinamide riboside (NR) is a form of vitamin B3 and is one of the most studied compounds for the restoration of cellular NAD+ levels demonstrating clinical potential in many metabolic and age-related disorders. Despite its wide commercial availability as a powerful nutraceutical, our understanding of NR uptake by different cells and tissues is greatly limited by the lack of noninvasive in vivo imaging tools limiting its clinical translation. Here, we report the development and validation of a bioluminescent NR uptake probe (BiNR) for non-invasive longitudinal imaging of NR uptake both in vitro and in vivo. In addition, we optimized an assay that allows monitoring of NR flux without the need to transfect cells with the luciferase gene, enabling the use of the BiNR probe in clinical samples, as demonstrated with human T cells. Lastly, we used BiNR to investigate the role of NR uptake in cancer prevalence and metastases formation in triple negative breast cancer (TNBC) animal model. Our results demonstrate that NR supplementation results in a significant increase in cancer prevalence and metastases of TNBC to the brain. These results outline the important role of powerful nutraceuticals like NR in cancer metabolism and the need to personalize their use in certain patient populations.
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