Rassf7a promotes spinal cord regeneration and controls spindle orientation in neural progenitor cells

Article21 November 2022 Transparent process Rassf7a promotes spinal cord regeneration and controls spindle orientation in neural progenitor cells Panpan Zhu Panpan Zhu orcid.org/0000-0001-8966-9402 Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China Contribution: Data curation, Software, Formal analysis, Validation, ​Investigation, Visualization, Methodology, Writing - original draft Search for more papers by this author Pengfei Zheng Pengfei Zheng orcid.org/0000-0002-8729-0850 Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Contribution: Data curation, Software, Formal analysis, Validation, Visualization, Methodology Search for more papers by this author Xinlong Kong Xinlong Kong orcid.org/0000-0003-1489-1054 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Contribution: Validation, Visualization, Methodology Search for more papers by this author Shuo Wang Shuo Wang orcid.org/0000-0001-8327-512X Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Contribution: Software, ​Investigation, Visualization, Methodology Search for more papers by this author Muqing Cao Corresponding Author Muqing Cao [email protected] orcid.org/0000-0002-0352-6548 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Contribution: Funding acquisition, Methodology, Writing - original draft, Writing - review & editing Search for more papers by this author Chengtian Zhao Corresponding Author Chengtian Zhao [email protected] orcid.org/0000-0003-1236-914X Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China Contribution: Conceptualization, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review & editing Search for more papers by this author Panpan Zhu Panpan Zhu orcid.org/0000-0001-8966-9402 Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China Contribution: Data curation, Software, Formal analysis, Validation, ​Investigation, Visualization, Methodology, Writing - original draft Search for more papers by this author Pengfei Zheng Pengfei Zheng orcid.org/0000-0002-8729-0850 Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Contribution: Data curation, Software, Formal analysis, Validation, Visualization, Methodology Search for more papers by this author Xinlong Kong Xinlong Kong orcid.org/0000-0003-1489-1054 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Contribution: Validation, Visualization, Methodology Search for more papers by this author Shuo Wang Shuo Wang orcid.org/0000-0001-8327-512X Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Contribution: Software, ​Investigation, Visualization, Methodology Search for more papers by this author Muqing Cao Corresponding Author Muqing Cao [email protected] orcid.org/0000-0002-0352-6548 Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China Contribution: Funding acquisition, Methodology, Writing - original draft, Writing - review & editing Search for more papers by this author Chengtian Zhao Corresponding Author Chengtian Zhao [email protected] orcid.org/0000-0003-1236-914X Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China Contribution: Conceptualization, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review & editing Search for more papers by this author Author Information Panpan Zhu1,2,3,†, Pengfei Zheng1,†, Xinlong Kong4, Shuo Wang1, Muqing Cao *,4 and Chengtian Zhao *,1,2,3 1Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China 2Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China 3Sars-Fang Centre, Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China 4Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China † These authors contributed equally to this work *Corresponding author. Tel: +86 21 63846590 776935; E-mail: [email protected] author. Tel: +86 532 8203 2962; E-mail: [email protected] EMBO Reports (2023)24:e54984https://doi.org/10.15252/embr.202254984 Full textView the full text of the articlePDFDownload PDF of article text and main figures.PDF PLUSDownload PDF of article text, main figures, expanded view figures and appendix. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions Figures & Info Abstract Spinal cord injury (SCI) can cause long-lasting disability in mammals due to the lack of axonal regrowth together with the inability to reinitiate spinal neurogenesis at the injury site. Deciphering the mechanisms that regulate the proliferation and differentiation of neural progenitor cells is critical for understanding spinal neurogenesis after injury. Compared with mammals, zebrafish show a remarkable capability of spinal cord regeneration. Here, we show that Rassf7a, a member of the Ras-association domain family, promotes spinal cord regeneration after injury. Zebrafish larvae harboring a rassf7a mutation show spinal cord regeneration and spinal neurogenesis defects. Live imaging shows abnormal asymmetric neurogenic divisions and spindle orientation defects in mutant neural progenitor cells. In line with this, the expression of rassf7a is enriched in neural progenitor cells. Subcellular analysis shows that Rassf7a localizes to the centrosome and is essential for cell cycle progression. Our data indicate a role for Rassf7a in modulating spindle orientation and the proliferation of neural progenitor cells after spinal cord injury. Synopsis Rassf7a locates to the spindle pole and orchestrates spindle rotation during cell division of neural progenitor cells. Rassf7a deficiency results in spindle orientation defects characterized by increased rotation angles and decreased perpendicular (asymmetric) cell divisions after spinal cord injury. Rassf7a is enriched in the neural progenitor cells. Zebrafish rassf7a mutants exhibit spinal cord regeneration and spinal neurogenesis defects. Rassf7a modulates spindle orientation and the proliferation of neural progenitor cells after spinal cord injury. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 24,Issue 1,09 January 2023This month's cover highlights the article Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM‐1 by Max Grönloh, Janine Arts, Jaap van Buul and colleagues. This is a confocal microscopic image of a whole-mount stain, originating from a patient suffering from inflammatory bowel disease. A small venule with a diameter of around 20 micron is highlighted. The nuclei of endothelial cells and cells surrounding the venule are shown in blue, F-actin is displayed in green and cell junctions are shown via a PECAM-1 stain in magenta. In red, ICAM-1, which is upregulated in such inflamed venules in a heterogeneous manner, giving rise to neutrophil transendothelial migration hotspots, is stained. (Cover concept by the authors; scientific illustration by Max Grönloh, Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Medical Center, Amsterdam, The Netherlands. The image was taken by Max Grönloh on a Zeiss LSM 980 Airyscan microscope. Copyright (author or Institute): Max Grönloh, Amsterdam UMC, location AMC.Scistories LLC.) Volume 24Issue 19 January 2023In this issue ReferencesRelatedDetailsLoading ...