毫秒
帧速率
显微镜
显微镜
穿透深度
吞吐量
双光子激发显微术
光学
计算机科学
薄层荧光显微镜
材料科学
扫描共焦电子显微镜
物理
荧光
天文
电信
无线
作者
Shifu Xiao,JOHN GIBLIN,David A. Boas,Jérôme Mertz
出处
期刊:Optica
[The Optical Society]
日期:2023-06-15
卷期号:10 (6): 763-763
被引量:2
标识
DOI:10.1364/optica.487272
摘要
High-speed laser scanning microscopes are essential for monitoring fast biological phenomena. However, existing strategies that achieve millisecond time resolution with two-photon microscopes (2PMs) are generally technically challenging and suffer from compromises among imaging field of view, excitation efficiency, and depth penetration in thick tissue. Here, we present a versatile solution that enables a conventional video-rate 2PM to perform 2D scanning at kilohertz frame rates over large fields of view. Our system is based on implementation of a scan multiplier unit that provides inertia-free multiplication of the scanning speed while preserving all the benefits of standard 2PM. We demonstrate kilohertz subcellular-resolution 2PM imaging with an order of magnitude higher imaging throughput than previously achievable and penetration depths exceeding 500 µm, which we apply to the study of neurovascular coupling dynamics in the mouse brain.
科研通智能强力驱动
Strongly Powered by AbleSci AI