费斯特共振能量转移
纳米棒
化学
猝灭(荧光)
光化学
生物传感器
葡萄糖氧化酶
量子点
荧光
接受者
分析化学(期刊)
材料科学
纳米技术
有机化学
物理
量子力学
凝聚态物理
作者
Yogyata Chawre,Manmohan L. Satnami,Ankita B. Kujur,Kallol K. Ghosh,Rekha Nagwanshi,Indrapal Karbhal,Shamsh Pervez,Manas Kanti Deb
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2023-04-27
卷期号:6 (9): 8046-8058
被引量:14
标识
DOI:10.1021/acsanm.3c01518
摘要
Multicolor emissive nitrogen-doped carbon quantum dots (CQDs) were synthesized by the hydrothermal method and characterized using spectroscopic and electron microscopic techniques. Förster resonance energy transfer (FRET) between CQDs and gold nanorods (AuNRs) has been investigated that occurs due to the distinctive overlap of transverse and longitudinal bands of AuNRs with the fluorescence (FL) spectra of CQDs. Significant FL quenching (turn-off) of CQDs by AuNRs and their recovery (turn-on) have been observed due to etching of AuNRs and end-to-end assemblies that lead to the detection of biomolecules like H2O2, glucose, glutathione (GSH), and acetylcholinesterase (AChE). The redox reaction of Au(0) with H2O2 results in the decomposition of AuNRs to give Au(I) ions, thereby inducing the fluorescence recovery of CQDs (turn-on). The interruption of the FRET phenomenon by the production of H2O2 from the reaction of glucose oxidase in the presence of glucose and a thiol-containing compound from the reaction of acetylthiocholine and the AChE enzyme causes the FL recovery of CQDs, respectively. Moreover, the assembly of AuNRs leads to the FRET disruption, and FL turn-on signals were found to be measures of GSH. In comparison to the UV–visible approach, the FL measurements through the FRET process are found to be more sensitive under the same reaction conditions. The practical applicability of the proposed sensing system has been verified using human plasma samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI