微通道
微流控
材料科学
吞吐量
流速
流动聚焦
炸薯条
实验室晶片
纳米技术
流量(数学)
生物医学工程
光电子学
计算机科学
工程类
机械
电信
物理
无线
作者
Xun Liu,J Zhou,Ruopeng Yan,Tao Tang,Shubin Wei,Rubing Li,Dan Hou,Yueyun Weng,Du Wang,Hui Shen,Fuling Zhou,Yo Tanaka,Ming Li,Yoichiroh Hosokawa,Yaxiaer Yalikun,Cheng Lei
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:23 (16): 3571-3580
被引量:3
摘要
Imaging flow cytometry (IFC) is a powerful tool for cell detection and analysis due to its high throughput and compatibility in image acquisition. Optical time-stretch (OTS) imaging is considered as one of the most promising imaging techniques for IFC because it can realize cell imaging at a flow speed of around 60 m s-1. However, existing PDMS-based microchannels cannot function at flow velocities higher than 10 m s-1; thus the capability of OTS-based IFC is significantly limited. To overcome the velocity barrier for PDMS-based microchannels, we proposed an optimized design of PDMS-based microchannels with reduced hydraulic resistance and 3D hydrodynamic focusing capability, which can drive fluids at an ultra-high flow velocity (of up to 40 m s-1) by using common syringe pumps. To verify the feasibility of our design, we fabricated and installed the microchannel in an OTS IFC system. The experimental results first proved that the proposed microchannel can support a stable flow velocity of up to 40 m s-1 without any leakage or damage. Then, we demonstrated that the OTS IFC is capable of imaging cells at a velocity of up to 40 m s-1 with good quality. To the best of our knowledge, it is the first time that IFC has achieved such a high flow velocity just by using a PDMS-glass chip. Moreover, high velocity can enhance the focusing of cells on the optical focal plane, increasing the number of detected cells and the throughput. This work provides a promising solution for IFC to fully release its capability of advanced imaging techniques by operating at an extremely high screening throughput.
科研通智能强力驱动
Strongly Powered by AbleSci AI