溴虫腈
菜蛾
生物
恶二唑虫
遗传学
菜蛾科
小菜蛾
交叉电阻
多杀菌素
阿维菌素
拉伤
人口
抗药性
植物
杀虫剂
幼虫
农学
人口学
解剖
社会学
作者
Qian Cheng,Jia Li,Shuwen Wu,Yihua Yang,Yidong Wu,Xingliang Wang
标识
DOI:10.1111/1744-7917.13249
摘要
Abstract Chlorfenapyr is a broad‐spectrum halogenated pyrrole insecticide with a unique mode of action. Due to the misuse and overuse of this chemical, resistance has been reported in several arthropods, including Plutella xylostella , which is one of the most destructive insect pests afflicting crucifers worldwide. A better understanding of the cross‐resistance and genetics of field‐evolved chlorfenapyr resistance could effectively guide resistance management practices. Here, the chlorfenapyr resistance of a field‐derived population of P. xylostella was introgressed into the susceptible IPP‐S strain using a selection‐assisted multigenerational backcrossing approach. The constructed near‐isogenic strain, TH‐BC 5 F 2 , shared 98.4% genetic background with the recurrent parent IPP‐S strain. The TH‐BC 5 F 2 strain showed 275‐fold resistance to chlorfenapyr, but no significant cross‐resistance to spinosad, abamectin, chlorpyrifos, β‐cypermethrin, indoxacarb, chlorantraniliprole, or broflanilide (no more than 4.2‐fold). Genetic analysis revealed that resistance was autosomal, incompletely dominant, and conferred by 1 major gene or a few tightly linked loci. The synergism of metabolic inhibitors (PBO, DEM, and DEF) to chlorfenapyr was very weak (<1.7‐fold), and the metabolic enzyme activities in the TH‐BC 5 F 2 strain were not significantly elevated compared with the IPP‐S strain. The results enhances our understanding of the genetic traits of chlorfenapyr resistance, and provides essential information for improving resistance management strategies.
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