背景(考古学)
胰岛素
油酸
脂肪酸
脂肪组织
生物
脂肪生成
油红O
化学
内科学
内分泌学
生物化学
医学
古生物学
作者
Wen Tian,Hua Xiang,Qian Li,Yong Wang,Jiangjiang Zhu,Yaqiu Lin
摘要
Context Oleic acid together with insulin is widely used to induce preadipocyte differentiation in humans and mammals, and is also used alone in chicken preadipocytes from abdominal adipose tissue. However, it is not clear whether oleic acid alone promotes goat primary intramuscular preadipocyte differentiation. Aims The aim of the present study was to identify the role of oleic acid in regulating primary preadipocyte differentiation in goats. Methods Three healthy, 7-day-old Jianzhou goats were randomly selected. After slaughter, the longissimus dorsi tissues were collected from each goat under sterile procedures and mixed equally. The primary preadipocytes were then prepared using collagenase type I digestion, and treated with 5 mg/L insulin or different concentrations of oleic acid, including 0 μM, 50 μM, 100 μM, 150 μM and 300 μM. The results were determined using microscopy and Oil Red O staining. The expression of genes related to preadipocyte differentiation were determined by real-time fluorescence quantitative polymerase chain reaction. Results Lower concentrations of oleic acid (50 μM, 100 μM and 150 μM) did not affect the cell morphology and cell growth, whereas 300 μM oleic acid led to severe cytotoxicity compared with the control (0 μM). The treatment of oleic acid (100 μM) enhanced cellular accumulation and lipid droplets deposition significantly, which was not affected by supplementary insulin. In addition, insulin alone treatment did not alter cellular adipogenesis in goat intramuscular preadipocytes. Treatment with oleic acid significantly increased the expression of peroxisome proliferator-activated receptor gamma, CCAAT enhancer-binding protein alpha and fatty acid binding protein 4, and decreased the expression of lipoprotein esterase on Day 2 after cell differentiation, all of which decreased continually on Day 4 and Day 6. Expression of all genes increased significantly on Day 8 after oleic acid treatment in goat intramuscular preadipocytes. Conclusion The results underscore the role of oleic acid independent of insulin in promoting intramuscular preadipocytes in goats, and probably via the control of peroxisome proliferator-activated receptor gamma and CCAAT enhancer-binding protein alpha. Implications These data provide insight into the mechanism underlying preadipocyte differentiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI