异源表达
聚酮
链霉菌
生物
基因簇
基因
辣椒疫霉
异源的
基因组
遗传学
计算生物学
细菌
生物合成
疫霉菌
重组DNA
植物
作者
Yu Liu,Zifei Xu,Ping Chen,Zuo Chen,Liyifan Chen,Wei Yan,Rui‐Hua Jiao,Yonghao Ye
标识
DOI:10.1021/acs.jafc.2c06928
摘要
A type II polyketide synthase biosynthetic gene cluster (nap) was identified in Streptomyces eurocidicus CGMCC 4.1086 via genome mining. The heterologous expression of the cryptic nap gene cluster in Streptomyces albus J1074 generated dimerized aromatic polyketide naphthocyclinones (1–3), whose structures were determined via extensive analysis using nuclear magnetic resonance and high-resolution electrospray ionization mass spectroscopy. The biological pathway of naphthocyclinone synthesis was revealed via in vivo gene deletion, in vitro biochemical reactions, and comparative genomics. Remarkably, 3 played a crucial role in inhibiting Phytophthora capsici and Phytophthora sojae, with EC50 values of 6.1 and 20.2 μg/mL, respectively. Furthermore, 3 exhibited a potent protective effect against P. capsici and P. sojae in greenhouse tests.
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