Face-to-face Assembly Strategy of Au Nanocubes: Induced Generation of Broad Hotspot Regions for SERS-Fluorescence Dual-Signal Detection of Intracellular miRNAs

化学 荧光 细胞内 小RNA 热点(地质) 对偶(语法数字) 纳米技术 生物物理学 细胞生物学 生物化学 光学 基因 艺术 物理 材料科学 文学类 地球物理学 生物 地质学
作者
Jiwei Wang,Shuo Ma,Kezhen Ge,Ran Xu,Fuzhi Shen,Xun Gao,Yuming Yao,Yaya Chen,Yuxin Chen,Fenglei Gao,Guoqiu Wu
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:96 (22): 8922-8931 被引量:23
标识
DOI:10.1021/acs.analchem.3c05743
摘要

While designing anisotropic noble metal nanoparticles (NPs) can enhance the signal intensity of Raman dyes, more sensitive surface-enhanced Raman scattering (SERS) probes can be designed by oriented self-assembly of noble metal nanomaterials into dimers or higher-order nanoclusters. In this study, we engineered a self-assembly strategy in living cells for real-time fluorescence and SERS dual-channel detection of intracellular microRNAs (miRNAs), using Mg2+-dependent 8–17E DNAzyme sequences as the driving motors, gold nanocubes (AuNCs) as the driver components, and three-branched double-stranded DNA as the linking tool. The assembly selects adenine in DNA as a reporter molecule, simplifying the labeling process of Raman reporter molecules and reducing the synthesis process. In addition, adenine is stably distributed between the faces of AuNCs and the wide hotspot region gives good reproducibility of the adenine SERS signal. In this strategy, the SERS channel was consistently stable and more sensitive compared to the fluorescence channel. Among them, the detection limit of the SERS channel was 2.1 pM and the coefficient of variation was 1.26% in the in vitro liquid phase and 1.49% in MCF-7 cells. The strategy successfully achieved accurate tracking and quantification of miRNA-21 in cancer cells, showing good reproducibility in complex samples as well as cells. The reported strategy provides ideas for exploring intracellular specific triggering of nanoparticles for precise control of self-assembly.
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