生物
小干扰RNA
小孢子
基因沉默
拟南芥
表观遗传学
细胞生物学
DNA甲基化
减数分裂
花粉
遗传学
生物发生
反式siRNA
有丝分裂
核糖核酸
基因表达
基因
突变体
植物
雄蕊
作者
Kannan Pachamuthu,Matthieu Simon,Filipe Borges
标识
DOI:10.1038/s41467-024-48950-6
摘要
Abstract In plants, small-interfering RNAs (siRNAs) mediate epigenetic silencing via the RNA-directed DNA methylation (RdDM) pathway, which is particularly prominent during reproduction and seed development. However, there is limited understanding of the origins and dynamics of reproductive siRNAs acting in different cellular and developmental contexts. Here, we used the RNaseIII-like protein RTL1 to suppress siRNA biogenesis in Arabidopsis pollen, and found distinct siRNA subsets produced during pollen development. We demonstrate that RTL1 expression in the late microspore and vegetative cell strongly impairs epigenetic silencing, and resembles RdDM mutants in their ability to bypass interploidy hybridization barriers in the seed. However, germline-specific RTL1 expression did not impact transgenerational inheritance of triploid seed lethality. These results reveal the existence of multiple siRNA subsets accumulated in mature pollen, and suggest that mobile siRNAs involved in the triploid block are produced in germline precursor cells after meiosis, or in the vegetative cell during pollen mitosis.
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