Comparative physiological and transcriptome analysis reveals the potential mechanism of selenium accumulation and tolerance to selenate toxicity of Broussonetia papyrifera

构属 硒酸盐 化学 转录组 生物化学 类黄酮 基因 生物 基因表达 抗氧化剂 植物 有机化学
作者
Qiangwen Chen,Yu Li,Wei Chao,Juan Xiang,Xiaoyan Yang,Jiabao Ye,Xiaoli Liao,Xian Zhou,Shen Rao,Shuiyuan Cheng,Xin Cong,Bo Xiao,Feng Xu
出处
期刊:Tree Physiology [Oxford University Press]
卷期号:42 (12): 2578-2595 被引量:8
标识
DOI:10.1093/treephys/tpac095
摘要

Broussonetia papyrifera is an important fodder tree that is widely distributed in China. Enhancing the selenium (Se) content in B. papyrifera may help to improve the nutritional value of the feed. In this study, sodium selenite and selenate were foliar applied to investigate the mechanisms of Se tolerance and accumulation in B. papyrifera. The results showed that both Se forms significantly increased the total Se content, and the proportion of organic Se was significantly higher in the sodium selenite treatment than in the control. In addition, the soluble sugar, phenolic acid and flavonoid contents and antioxidant enzyme activities were increased by exogenous Se. The de novo RNA sequencing results showed that 644 and 1804 differentially expressed genes were identified in the selenite and selenate comparison groups, respectively. Pathway enrichment analysis demonstrated that 24 of the 108 pathways were significantly enriched, of which sulfur assimilation genes in the sodium selenite-treated groups were upregulated, whereas Se conjugation and transporter genes, such as SBP1, PCS, GSTs, ABCs and GPX, were significantly induced under selenate treatment. The hub genes identified by weighted-gene co-expression network analysis further confirmed that sulfur assimilation, conjugation and transporter genes might play a vital role in Se assimilation and tolerance. From this, a model of Se metabolism in B. papyrifera was proposed based on the above physiological and RNA sequencing data. This study is the first study to report that B. papyrifera has a strong ability to accumulate and tolerate exogenous Se, thereby providing a foundation for further characterization of the accumulation and tolerance mechanism of B. papyrifera. Our findings can provide technical support for producing Se-enriched fodder.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
nanfeng完成签到 ,获得积分10
10秒前
12秒前
15秒前
金光一闪发布了新的文献求助10
19秒前
狼来了aas发布了新的文献求助10
21秒前
rumengzhuo完成签到,获得积分10
23秒前
gyx完成签到 ,获得积分10
26秒前
金光一闪完成签到,获得积分10
26秒前
二十八完成签到 ,获得积分10
38秒前
zx完成签到 ,获得积分10
40秒前
彩色的芷容完成签到 ,获得积分10
40秒前
乐观的星月完成签到 ,获得积分10
44秒前
eternal_dreams完成签到 ,获得积分10
48秒前
西安浴日光能赵炜完成签到,获得积分10
1分钟前
vagabond完成签到 ,获得积分10
1分钟前
1分钟前
XQ完成签到,获得积分10
1分钟前
Wang发布了新的文献求助10
1分钟前
liars完成签到 ,获得积分10
1分钟前
juliar完成签到 ,获得积分10
1分钟前
迅速千愁完成签到 ,获得积分10
1分钟前
fawr完成签到 ,获得积分10
1分钟前
一颗西柚完成签到 ,获得积分10
1分钟前
唐唐完成签到,获得积分10
1分钟前
strama完成签到,获得积分10
1分钟前
苏菲完成签到 ,获得积分10
1分钟前
酷波er应助科研通管家采纳,获得10
1分钟前
Sally完成签到 ,获得积分10
2分钟前
阳炎完成签到,获得积分10
2分钟前
欣忆完成签到 ,获得积分10
2分钟前
共享精神应助hipig采纳,获得10
2分钟前
Tina完成签到 ,获得积分10
2分钟前
现代的紫霜完成签到,获得积分10
2分钟前
快去爬山完成签到 ,获得积分10
2分钟前
东方越彬发布了新的文献求助10
2分钟前
杪夏二八完成签到 ,获得积分10
2分钟前
咖啡味椰果完成签到 ,获得积分10
2分钟前
2分钟前
满满的都是橙汁完成签到,获得积分10
2分钟前
Axs完成签到,获得积分10
2分钟前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3571332
求助须知:如何正确求助?哪些是违规求助? 3141926
关于积分的说明 9444874
捐赠科研通 2843331
什么是DOI,文献DOI怎么找? 1562830
邀请新用户注册赠送积分活动 731326
科研通“疑难数据库(出版商)”最低求助积分说明 718524