碘化丙啶
活性氧
细胞毒性
二氯荧光素
银纳米粒子
膜联蛋白
氧化应激
化学
坏死
细胞凋亡
纳米颗粒
生物物理学
超氧化物歧化酶
核化学
程序性细胞死亡
分子生物学
体外
活力测定
超氧化物
材料科学
生物化学
纳米技术
生物
遗传学
作者
Rasmus Foldbjerg,Ping Olesen,Mads Hougaard,Duy Anh Dang,Hans Jürgen Hoffmann,Herman Autrup
标识
DOI:10.1016/j.toxlet.2009.07.009
摘要
The objective of the present study was to investigate the toxicity of silver nanoparticles (Ag NPs) in vitro. Silver ions (Ag+) have been used in medical treatments for decades whereas Ag NPs have been used in a variety of consumer products within recent years. This study was undertaken to compare the effect of well characterized, PVP-coated Ag NPs (69 nm ± 3 nm) and Ag+ in a human monocytic cell line (THP-1). Characterization of the Ag NPs was conducted in both stock suspension and cell media with or without serum and antibiotics. By using the flowcytometric annexin V/propidium iodide (PI) assay, both Ag NPs and Ag+ were shown to induce apoptosis and necrosis in THP-1 cells depending on dose and exposure time. Furthermore, the presence of apoptosis could be confirmed by the TUNEL method. A number of studies have implicated the production of reactive oxygen species (ROS) in cytotoxicity mediated by NPs. We used the fluorogenic probe, 2′,7′-dichlorofluorescein to assess the levels of intracellular ROS during exposure to Ag NPs and Ag+. A drastic increase in ROS levels could be detected after 6–24 h suggesting that oxidative stress is an important mediator of cytotoxicity caused by Ag NPs and Ag+.
科研通智能强力驱动
Strongly Powered by AbleSci AI