英特因
蛋白质剪接
化学生物学
计算生物学
背景(考古学)
蛋白质工程
RNA剪接
生物
化学
生物化学
基因
酶
古生物学
核糖核酸
作者
Neel H. Shah,Tom W. Muir
出处
期刊:Chemical Science
[The Royal Society of Chemistry]
日期:2014-01-01
卷期号:5 (2): 446-461
被引量:340
摘要
Inteins are auto-processing domains found in organisms from all domains of life. These proteins carry out a process known as protein splicing, which is a multi-step biochemical reaction comprised of both the cleavage and formation of peptide bonds. While the endogenous substrates of protein splicing are specific essential proteins found in intein-containing host organisms, inteins are also functional in exogenous contexts and can be used to chemically manipulate virtually any polypeptide backbone. Given this, protein chemists have exploited various facets of intein reactivity to modify proteins in myriad ways for both basic biological research as well as potential therapeutic applications. Here, we review the intein field, first focusing on the biological context and phylogenetic diversity of inteins, followed by a description of intein structure and biochemical function. Finally, we discuss prevalent inteinbased technologies, focusing on their applications in chemical biology, followed by persistent caveats of intein chemistry and approaches to alleviate these shortcomings. The findings summarized herein describe two and a half decades of research, leading from a biochemical curiosity to the development of powerful protein engineering tools.
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