腹水
卵巢癌
小RNA
转移
癌症研究
液体活检
癌症
胞外囊泡
医学
生物
病理
内科学
微泡
基因
生物化学
作者
Wenyu Wang,HyunA Jo,Sangick Park,Heeyeon Kim,Se Ik Kim,Youngjin Han,Juwon Lee,Aeran Seol,Junhwan Kim,Maria Lee,Chul Lee,Danny N. Dhanasekaran,Tae-Jin Ahn,Yong Sang Song
出处
期刊:Cancer Letters
[Elsevier]
日期:2022-08-01
卷期号:542: 215735-215735
被引量:41
标识
DOI:10.1016/j.canlet.2022.215735
摘要
Ovarian cancer is mostly diagnosed at advantaged stages due to the lack of early diagnostic biomarkers. The common metastasis pattern is characterized by peritoneal dissemination with a formation of malignant ascites. Extracellular vesicles (EVs) are emerging as promising clinical biomarkers in liquid biopsy. Here, we aimed to investigate robust liquid biopsy-based EV miRNA biomarkers for ovarian cancer diagnosis and metastasis regulation. EVs were isolated from malignant ascites and plasma of ovarian cancer patients as well as the benign control counterparts of patients with benign gynecologic diseases. EV small RNA sequencing identified a panel of eight miRNAs (miR-1246, miR-1290, miR-483, miR-429, miR-34b-3p, miR-34c-5p, miR-145–5p, miR-449a) based on dysregulated miRNAs overlapped in the ascites and plasma subset. The ovarian cancer EV miRNA (OCEM) signature developed based on these eight miRNAs demonstrated high diagnostic accuracy in our in-house dataset and multiple public datasets across diverse clinical samples (blood, tissue and urine). In addition, malignant ascites-derived EVs could significantly facilitate the aggressive property of ovarian cancer cells and boost the growth of ascites-derived organoids. Notably, miR-1246 and miR-1290 shuttled in malignant ascites-derived EVs were identified to promote the invasion and migration of ovarian cancer cells through regulating a common target RORα.
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