亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Retinoic acid promotes formation of chicken (Gallus gallus) spermatogonial stem cells by regulating the ECM-receptor interaction signaling pathway

生物 转录组 细胞生物学 信号转导 转录因子 整合素 基因 流式细胞术 受体 基因表达 分子生物学 遗传学
作者
Cai Hu,Qisheng Zuo,Kai Jin,Zhehong Zhao,Yuhui Wu,Gao Ji-chang,Chaoyong Wang,Yingjie Wang,Wanda Zhan,Jing Zhou,Fufu Cheng,Hongyan Sun,Yingjie Niu,Yani Zhang
出处
期刊:Gene [Elsevier]
卷期号:820: 146227-146227 被引量:15
标识
DOI:10.1016/j.gene.2022.146227
摘要

Spermatogonial stem cells (SSCs) are the basis of spermatogenesis. Systematically exploring the critical factors associated with the formation of SSCs will provide new insight to improve the formation efficiency, and their practical application. Here we explore the regulatory mechanism of the ECM-receptor interaction signaling pathway and related genes during differentiation of SSCs in chicken. Firstly, the positive cell rate of SSCs protein marker was detected by immunofluorescence and flow cytometry and qRT-PCR was used to identify, the expression of related marker genes after 10 days of RA-induction. Secondly, the ESCs on 0d/ 4d /10d after RA- induction/self-differentiation were collected, and the total RNA was then extracted from cells. Finally, high-throughput analysis methods (RNA-seq) were used to sequence the transcriptome of these cells. After PCA analysis of the RNA-seq data, Venny analysis, GO and KEGG enrichment were further used to find the key signaling pathways and genes in the RA-induction process. The results showed that on day 10 of RA-induction, grape cluster growth cells expressed integrinβ1, the specific marker protein of SSCs cells, and the integrinβ1 positive rate was 35.1%. Also, SSCs marker genes CVH, Integrinβ1, Integrinα6 were significantly up-regulated during RA-induction. Moreover, the significantly enriched pathway, ECM-receptor interaction signaling, in current study may play a crucial role in RA-induction. Then, JASPAR was used to predict the differential gene transcription factors in the signaling pathway, finding that RA receptor was a transcription factor of COL5A1, COL5A2 and COL3A1. The qRT-PCR results showed that the expression levels of RA receptors (RXRA, RARA and RXRG) and the predicted genes (COL5A1, COL5A2 and COL3A1) were both significantly increased during RA-induction. Also, dual-luciferase reporter assay showed that RA could affect the luciferin activities of COL5A1, COL5A2 and COL3A1. These results suggest that RA plays a crucial role in the formation of chicken spermatogonial stem cells via the transcription levels of COL5A1, COL5A2 and COL3A1 to regulate the ECM-receptor interaction signaling pathway. Additionally, knockdown of COL5A1/COL5A2/COL3A1 could effectively reduce the formation efficiency of SSCs. This indicated that the interference of RA receptor binding genes in the ECM-receptor interaction signaling pathway could decrease the efficiency of RA induced SSCs formation. Therefore, this study concludes that RA promotes formation of chicken spermatogonial stem cells by regulating the ECM-receptor interaction signaling pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
高小羊完成签到,获得积分10
2秒前
LouieHuang完成签到,获得积分20
52秒前
1分钟前
郜南烟发布了新的文献求助10
1分钟前
wanci应助郜南烟采纳,获得10
1分钟前
上官若男应助zhangyimg采纳,获得10
1分钟前
1分钟前
Lorin完成签到 ,获得积分10
1分钟前
1分钟前
zhangyimg发布了新的文献求助10
1分钟前
科目三应助zhangyimg采纳,获得10
2分钟前
gszy1975完成签到,获得积分10
2分钟前
圆圆的波仔发布了新的文献求助100
2分钟前
JamesPei应助科研通管家采纳,获得10
3分钟前
郗妫完成签到,获得积分10
3分钟前
4分钟前
郜南烟发布了新的文献求助10
4分钟前
Venus完成签到 ,获得积分10
6分钟前
在水一方应助chenyuns采纳,获得30
6分钟前
JACk完成签到 ,获得积分10
6分钟前
6分钟前
chenyuns发布了新的文献求助30
6分钟前
爱静静应助李伟采纳,获得10
6分钟前
7分钟前
zhangyimg发布了新的文献求助10
7分钟前
7分钟前
郜南烟发布了新的文献求助10
7分钟前
斯文败类应助郜南烟采纳,获得10
8分钟前
思源应助chenyuns采纳,获得20
8分钟前
Akim应助chenyuns采纳,获得20
8分钟前
领导范儿应助圆圆的波仔采纳,获得10
9分钟前
9分钟前
9分钟前
李爱国应助怕孤单的灵寒采纳,获得10
9分钟前
圆圆的波仔完成签到,获得积分10
10分钟前
10分钟前
10分钟前
怕孤单的灵寒完成签到,获得积分20
10分钟前
10分钟前
chenyuns发布了新的文献求助20
10分钟前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
叶剑英与华南分局档案史料 500
Foreign Policy of the French Second Empire: A Bibliography 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3146771
求助须知:如何正确求助?哪些是违规求助? 2798063
关于积分的说明 7826621
捐赠科研通 2454573
什么是DOI,文献DOI怎么找? 1306394
科研通“疑难数据库(出版商)”最低求助积分说明 627708
版权声明 601527