Facile Purification and Concentration of DNA Origami Structures by Ethanol Precipitation

Abstract As a powerful self‐assembly strategy, DNA origami technique has found numerous applications in various areas. Many of the applications require pure and dense DNA origami solutions, therefore, the post‐assembly treatment of purification and concentration is essentially required. Herein, we reported a fast and facile approach for simultaneously purifying and concentrating DNA origami objects through ethanol precipitation. The triangular and rectangular DNA origami nanostructures have been successfully purified and concentrated using our approach. Our work provides a simple yet efficient way for preparing concentrated and purified DNA origami, which is expected to be applicable to the scalable production of pure and dense DNA origami solutions.