生物
分类单元
相对物种丰度
丰度(生态学)
微生物群
生态学
扩增子测序
微生物生态学
进化生物学
16S核糖体RNA
古生物学
细菌
生物信息学
作者
Ruben Props,Frederiek‐Maarten Kerckhof,Peter Rubbens,Jo De Vrieze,Emma Hernandez‐Sanabria,Willem Waegeman,Pieter Monsieurs,Frederik Hammes,Nico Boon
出处
期刊:The ISME Journal
[Springer Nature]
日期:2016-09-09
卷期号:11 (2): 584-587
被引量:283
标识
DOI:10.1038/ismej.2016.117
摘要
High-throughput amplicon sequencing has become a well-established approach for microbial community profiling. Correlating shifts in the relative abundances of bacterial taxa with environmental gradients is the goal of many microbiome surveys. As the abundances generated by this technology are semi-quantitative by definition, the observed dynamics may not accurately reflect those of the actual taxon densities. We combined the sequencing approach (16S rRNA gene) with robust single-cell enumeration technologies (flow cytometry) to quantify the absolute taxon abundances. A detailed longitudinal analysis of the absolute abundances resulted in distinct abundance profiles that were less ambiguous and expressed in units that can be directly compared across studies. We further provide evidence that the enrichment of taxa (increase in relative abundance) does not necessarily relate to the outgrowth of taxa (increase in absolute abundance). Our results highlight that both relative and absolute abundances should be considered for a comprehensive biological interpretation of microbiome surveys.
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