清脆的
Cas9
计算生物学
审问
基因组
基因组编辑
生物
德隆
基因
计算机科学
遗传学
历史
泛素
泛素连接酶
考古
作者
Basudeb Maji,Christopher Moore,Bernd Zetsche,Sara E. Volz,Feng Zhang,Matthew D. Shoulders,Amit Choudhary
标识
DOI:10.1038/nchembio.2224
摘要
Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.
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