二硫化钼
生物传感器
碳纳米管
纳米颗粒
胶体金
材料科学
电极
检出限
葡萄糖氧化酶
纳米技术
电化学
钼
石墨烯
纳米管
化学
色谱法
复合材料
物理化学
冶金
作者
Ke‐Jing Huang,Yujie Liu,Hai‐Bo Wang,Yaya Wang,Yan‐Ming Liu
标识
DOI:10.1016/j.bios.2013.11.061
摘要
A novel 2-dimensional graphene analog molybdenum disulfide/multi-walled carbon nanotube (MoS2/MWCNT) was synthesized by a simple hydrothermal method to achieve excellent electrochemical properties. An ultrasensitive electrochemical DNA biosensor was subsequently constructed by assembling a thiol-tagged DNA probe on a MoS2/MWCNT and gold nanoparticle (AuNP)-modified electrode that has already been coupled with glucose oxidase (GOD). In this work, GOD was used as a redox marker. The heteronanostructure formed on the biosensor surface appeared relatively good conductor for accelerating the electron transfer, while the modification of GOD and AuNPs provided multiple signal amplification for electrochemical biosensing. The multiple signal amplification strategy produced an ultrasensitive electrochemical detection of DNA down to 0.79 fM with a linear range from 10 fM to 107 fM, and appeared high selectivity to differentiate three-base mismatched DNA and one-base mismatched DNA. The developed approach provided a simple and reliable method for DNA detection with high sensitivity and specificity, and would open new opportunities for sensitive detection of other biorecognition events.
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