Fluorogenic DNAzyme Probes as Bacterial Indicators

Lighting up bacteria: An RNA-cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluorophore (F) and a quencher (Q). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.