In this paper, we intend to implement multi-scale texture segmentation by fractional differential. We propose two fractional differential masks and present the structures and parameters of each mask, respectively, on eight directions. Moreover, by theoretical and experimental analysis, we find the better performance fractional differential mask. Finally, we further discuss the capability of fractional differential for multi-scale texture segmentation. Experiments show that, for rich-grained digital images, the capability for multi-scale texture segmentation by fractional differential-based approach appears efficient.