染色
鉴别染色
核酸
污渍
动力学
化学
茜素红
分子生物学
核糖核酸
生物化学
生物
基因
遗传学
量子力学
物理
标识
DOI:10.1080/10520290312120006
摘要
AbstractSince the introduction of the methyl green-pyronin Y procedure as a differential histological stain more than 100 years ago, the method has become a histochemical procedure for differential demonstration of DNA and RNA. Numerous variants of the procedure have been suggested, and a number of hypotheses have been put forward concerning kinetics and binding mechanisms. Using both filter paper models containing DNA, RNA or heparin and histological sections, we have attempted to evaluate the kinetics of staining and the role of staining time for methyl green and pyronin Y by applying the dyes individually, simultaneously and sequentially. The results are presented as color charts approximating the observed staining patterns using a computerized palette. Our results indicate unequivocally that the differential staining is not time-dependent, but that it is dictated by the relative concentrations of methyl green and pyronin Y and by the pH of the staining solution.Keywordsdifferential stainingkineticsmethyl green-pyronin Ynucleic acid stainingrate model
科研通智能强力驱动
Strongly Powered by AbleSci AI