牙密螺旋体
中间普氏菌
连翘
放线杆菌
塔克曼
生物
牙龈卟啉单胞菌
微生物学
聚合酶链反应
牙周炎
实时聚合酶链反应
底漆(化妆品)
细菌
普雷沃菌属
病理
医学
牙科
基因
化学
遗传学
有机化学
中医药
替代医学
金银花
作者
Masae Kuboniwa,Atsuo Amano,K R Kimura,Shinichi Sekine,Shingo Kato,Yumiko Yamamoto,Nobuo Okahashi,Tetsuya Iida,Satoshi Shizukuishi
出处
期刊:Oral Microbiology and Immunology
[Wiley]
日期:2004-04-26
卷期号:19 (3): 168-176
被引量:171
标识
DOI:10.1111/j.0902-0055.2004.00135.x
摘要
Quantitative analysis, with identification of periodontopathic bacteria, is important for the diagnosis, therapeutic evaluation and risk assessment of periodontal disease. We developed a highly sensitive and specific method using real‐time polymerase chain reaction (PCR) to detect and quantify six periodontal bacteria: Porphyromonas gingivalis, Tannerella forsythia, Actinobacillus actinomycetemcomitans, Treponema denticola, Prevotella intermedia , and Prevotella nigrescens . Species‐specific TaqMan probe/primer sets were designed according to 16S ribosomal RNA gene sequences. Plaque and tongue debris specimens were collected from 10 patients with advanced periodontitis and 10 periodontal healthy individuals and analyzed. All species, except for P. nigrescens , were detected in samples from diseased sites in significantly greater numbers than in those from healthy sites, whereas greater numbers of P. nigrescens were found in the controls. These results suggest that the present real‐time PCR method with the designed probe/primer sets enabled sensitive detection of the six periodontal bacteria, and may also assist future microbial studies of periodontal diseases.
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