3‘-Ferrocene-Labeled Oligonucleotide Chains End-Tethered to Gold Electrode Surfaces: Novel Model Systems for Exploring Flexibility of Short DNA Using Cyclic Voltammetry

The flexibility of DNA is of central importance in biology, medicine, materials science, and mechanical engineering. In this study, we report an unprecedented electrochemical approach for investigating the flexibility of a short (typically 20-base), surface end-tethered single-stranded synthetic DNA oligonucleotide and of its postformed DNA duplex, taking as an example the homopolymer (dT)20 sequence in the regime of very high ionic strength ( approximately 1 M).