根毛
突变体
扩张素
生物
延伸率
细胞生物学
拟南芥
水稻
互补
基因
生物化学
基因表达
极限抗拉强度
冶金
材料科学
作者
Zhiming Yu,Bo Kang,Xiaowei He,Shaolei Lv,Youhuang Bai,Wona Ding,Ming Chen,Hyung‐Taeg Cho,Wu Ping
出处
期刊:Plant Journal
[Wiley]
日期:2011-02-18
卷期号:66 (5): 725-734
被引量:159
标识
DOI:10.1111/j.1365-313x.2011.04533.x
摘要
Summary Root hair growth requires intensive cell‐wall modification. This study demonstrates that root hair‐specific expansin As, a sub‐clade of the cell wall‐loosening expansin proteins, are required for root hair elongation in rice ( Oryza sativa L.). We identified a gene encoding EXPA17 ( OsEXPA17 ) from a rice mutant with short root hairs. Promoter::reporter transgenic lines exhibited exclusive OsEXPA17 expression in root hair cells. The OsEXPA17 mutant protein (OsexpA17) contained a point mutation, causing a change in the amino acid sequence (Gly104→Arg). This amino acid alteration is predicted to disrupt a highly conserved disulfide bond in the mutant. Suppression of OsEXPA17 by RNA interference further confirmed requirement for the gene in root hair elongation. Complementation of the OsEXPA17 mutant with other root hair EXPAs (OsEXPA30 and Arabidopsis EXPA7) can restore root hair elongation, indicating functional conservation of these root hair EXPAs in monocots and dicots. These results demonstrate that members of the root hair EXPA sub‐clade play a crucial role in root hair cell elongation in Graminaceae.
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