Single-cell analysis of cardiogenesis reveals basis for organ-level developmental defects

生物 心脏发育 祖细胞 细胞生物学 胚胎干细胞 转录组 转录因子 电池类型 祖细胞 单细胞分析 干细胞 细胞命运测定 细胞 遗传学 基因 基因表达
作者
T. Yvanka de Soysa,Sanjeev S. Ranade,Satoshi Okawa,Srikanth Ravichandran,Yu Huang,Hazel T. Salunga,Amelia Schricker,Antonio del Sol,Casey A. Gifford,Deepak Srivastava
出处
期刊:Nature [Nature Portfolio]
卷期号:572 (7767): 120-124 被引量:244
标识
DOI:10.1038/s41586-019-1414-x
摘要

Organogenesis involves integration of diverse cell types; dysregulation of cell-type-specific gene networks results in birth defects, which affect 5% of live births. Congenital heart defects are the most common malformations, and result from disruption of discrete subsets of cardiac progenitor cells1, but the transcriptional changes in individual progenitors that lead to organ-level defects remain unknown. Here we used single-cell RNA sequencing to interrogate early cardiac progenitor cells as they become specified during normal and abnormal cardiogenesis, revealing how dysregulation of specific cellular subpopulations has catastrophic consequences. A network-based computational method for single-cell RNA-sequencing analysis that predicts lineage-specifying transcription factors2,3 identified Hand2 as a specifier of outflow tract cells but not right ventricular cells, despite the failure of right ventricular formation in Hand2-null mice4. Temporal single-cell-transcriptome analysis of Hand2-null embryos revealed failure of outflow tract myocardium specification, whereas right ventricular myocardium was specified but failed to properly differentiate and migrate. Loss of Hand2 also led to dysregulation of retinoic acid signalling and disruption of anterior–posterior patterning of cardiac progenitors. This work reveals transcriptional determinants that specify fate and differentiation in individual cardiac progenitor cells, and exposes mechanisms of disrupted cardiac development at single-cell resolution, providing a framework for investigating congenital heart defects. Single-cell RNA-sequencing analysis reveals functions of lineage-specifying transcription factors underlying congenital defects in heart development.
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