Oxidative stress‐related biomarkers in saliva and gingival crevicular fluid associated with chronic periodontitis: A systematic review and meta‐analysis

Abstract Aim Oxidative stress ( OS ) biomarkers have been detected in saliva and gingival crevicular fluid ( GCF ) during chronic periodontitis ( CP ) progression; however, the relationship between OS biomarkers and CP progression remains elusive. The purpose of this meta‐analysis is to investigate the relationship between local OS biomarkers and CP . Methods This review was conducted through a systematic search from three databases. Studies on CP participants were included as an experimental group, and studies on periodontally healthy ( PH ) participants were included as a control. Mean effects were expressed as standardized mean difference with their associated 95% confidence intervals. Results From a total of 2,972 articles, 32 articles fulfilled the inclusion criteria. We found a significant decrease of total antioxidant capacity and a significant increase of malondialdehyde ( MDA ), nitric oxide, total oxidant status ( TOS ), and 8‐hydroxy‐deoxyguanosine levels in the saliva of CP patients. Moreover, we also found an elevation of MDA level in GCF of CP group when compared with the PH group. There were no significant differences of salivary and GCF superoxide dismutase levels, salivary glutathione peroxidase level, and GCF TOS level between two groups. However, a high heterogeneity was observed among evaluated studies. Conclusions Despite the limitations of this study, the result of our meta‐analysis supported the rationale that there was a direct link between CP and OS ‐related biomarkers’ levels in the local site, indicating the important role of OS in the onset and development of CP .