光热治疗
基因沉默
小干扰RNA
材料科学
热休克蛋白
体外
RNA干扰
体内
小发夹RNA
遗传增强
热休克蛋白27
生物物理学
转染
细胞生物学
纳米技术
癌症研究
细胞凋亡
热休克蛋白70
核糖核酸
医学
化学
基因敲除
生物
生物化学
生物技术
基因
作者
Yanan Liu,Meng Xu,Yingyu Zhao,Xu Chen,Xufeng Zhu,Chunfang Wei,Shuang Zhao,Jie Liu,Xiu‐Ying Qin
摘要
Due to the anti-apoptotic effect employed by cells to protect themselves, recent research shows that photothermal therapy (PTT) can lead to heat shock response, thus reducing the effect of treatment on cancer cells. Small interfering RNA (siRNA), as an effective carrier of RNA interference, can silence the expression of heat shock protein, HSPs or BAG3 genes by inhibiting the expression of specific genes, and thereby inhibiting heat shock response and making cancer cells more sensitive to PTT. In this study, flower-like gold nanoparticles were used as a core for a layer-by-layer strategy to produce a safe and biodegradable nanoparticle platform for gene silencing and photothermal therapy. The results showed that when the mass ratio of the GNFs and siRNA was 20 : 1, the loading efficiency was above 90%, which can effectively silence the expression of BAG3 siRNA. We demonstrated that the GNFs-siRNA still had a good photothermal effect after siRNA modification. In vitro, the GNFs-siRNA showed good biocompatibility and effectively tumor killing properties after laser irradiation. Furthermore, the GNFs-siRNA with laser treatment significantly decreased the expression of BAG3 and remarkably inhibited tumor growth in vivo. This nanosystem establishes an optimized platform for future gene delivery and photothermal therapy.
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