膜
小泡
色谱法
化学
等离子体
质子化
猝灭(荧光)
荧光
相(物质)
分析化学(期刊)
生物化学
离子
有机化学
量子力学
物理
作者
Anette Lund,Anja T. Fuglsang
标识
DOI:10.1007/978-1-61779-986-0_14
摘要
Purification of plasma membranes by two-phase partitioning is based on the separation of microsomal membranes, dependent on their surface hydrophobicity. Here we explain the purification of plasma membranes from a relatively small amount of material (7–30 g). The fluorescent probe ACMA (9-amino-6-chloro-2-metoxyacridine) accumulates inside the vesicles upon protonation. Quenching of ACMA in the solution corresponds to the H+ transport across the plasma membrane. Before running the assay, the plasma membranes are incubated with the detergent Brij-58 in order to create inside-out vesicles.
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