Key Technologies and Progress of Amperometric Biosensors Based on Dehydrogenases

Dehydrogenases,which use nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) as the coenzyme for their catalyzed reactions,are the most important grou Pof oxidoreductases in the nature.In recent years,Biosensors based on NAD(P)+-dependent dehydrogenases have been developed rapidly.Two key technologies are quite important for the construction of these biosensors.One is the electrochemical regeneration of oxidated form of coenzyme,and another is the coenzyme immobilization in electrode surface.Methods were reviewal to aid the electrochemical regeneration of coenzyme.Common ways of coenzyme immobilization were also introduced.In addition,research progress of the two key technologies was reviewed.