Study on the extraction of polysaccharides from male and female Korla pear pulps

Objective To study on the optimal extraction technology of polysaccharides in Xinjiang male and female Korla pear pulps.Methods Water as solvent,water bath reflux as extraction method,UV-visible spectrophotometry as determination method,and polysaccharide content as an indicator,the extraction temperature,solid-liquid ratio and extraction time were investigated.The detection wavelength was 621 nm.The regression equation was Y= 51.492 X-0.010 7,r=0.999 8.Results The polysaccharides in male pears optimal extraction conditions were as follows:liquid ratio of 1∶60,extraction time 180 min,extraction temperature 70 ℃,extraction times thrice.The optimal extraction conditions for polysaccharides in female pears were as follows:solidliquid ratio 1∶60,extraction time 60 min,extraction temperature 60 ℃,extraction times once.The average yield of polysaccharides of male and female pears were 70.03 and 78.29mg·g-1,respectively.Conclusion This method is simple,reproducible,sensitive and can be used to determine the total polysaccharides in Korla pears.