脂质体
贪婪
刀豆蛋白A
细胞毒性
Zeta电位
化学
等温滴定量热法
生物物理学
生物化学
分子生物学
体外
生物
免疫学
材料科学
纳米技术
抗原
纳米颗粒
作者
Nereide Stela Santos-Magalhães,Mariane Cajubá de Britto Lira Nogueira,Larissa Constantino França,Milena Sales Ferraz,Maria Clara Barros,Victor Passos Gibson,Francisco Xavier
出处
期刊:Anti-cancer Agents in Medicinal Chemistry
[Bentham Science]
日期:2022-03-01
卷期号:22 (5): 968-977
被引量:3
标识
DOI:10.2174/1871520621666210624112452
摘要
Target treatment using site-specific nanosystems is a hot topic for treating several diseases, especially cancer.The study was set out to develop site-specific liposomes using ConcanavalinA (ConA) to target β- lapachone(β-lap) to human breast cancer cells.Liposomes were prepared and characterized according to diameter size, zeta potential, ConA conjugation(%) and β-lap encapsulation efficiency (%). Isothermal Titration Calorimetry evaluated the binding energy between the biomolecules, which compose of the liposomes. ConA avidity was assessed before and after conjugation. Cytotoxicity was evaluated, and fluorescence microscopy was performed to investigate the influence of ConA influenced on MCF-7 uptake.Uncoated and ConA-coated liposomes presented size, and zeta potential values from 97.46 ± 2.01 to 152.23 ± 2.73 nm, and -6.83 ± 0.28 to -17.23 ±0.64 mV, respectively. Both ConA conjugation and β-lap encapsulation efficiency were approximately 100%. The favorable and spontaneous process confirmed the binding between ConA and the lipid. Hemagglutination assay confirmed ConA avidity once Lipo-ConA and Lipo-PEG-ConA were able to hemagglutinate the red blood cells at 128-1 and 256-1, respectively. Lipo-ConA was not cytotoxic, and the site-specific liposomes presented the highest toxicity. ConA-coated liposomes were more internalized by MCF7 than uncoated-liposomes.Therefore, the presence of ConA on the surface of liposomes influenced MCF7 uptake, in that way could be used as a promising site-specific system to target β-lap to cancer cells.
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