Antibacterial and antibiofilm effectiveness of bioactive packaging materials from edible sodium alginate and vanillin: Assessment on lettuce

Journal of Food Processing and PreservationVolume 45, Issue 9 e15668 ORIGINAL ARTICLE Antibacterial and antibiofilm effectiveness of bioactive packaging materials from edible sodium alginate and vanillin: Assessment on lettuce Shatabdi Das, Shatabdi Das Department of Microbiology, Techno India University, Kolkata, India Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Project administration, Validation, Visualization, Writing - original draftSearch for more papers by this authorKumari Vishakha, Kumari Vishakha Department of Microbiology, Techno India University, Kolkata, India Contribution: Data curation, Formal analysis, ​Investigation, MethodologySearch for more papers by this authorSatarupa Banerjee, Satarupa Banerjee Department of Microbiology, Techno India University, Kolkata, India Contribution: MethodologySearch for more papers by this authorSandhimita Mondal, Corresponding Author Sandhimita Mondal sandhimita@gmail.com Department of Microbiology, Techno India University, Kolkata, India Correspondence Arnab Ganguli and Sandhimita Mondal, Department of Microbiology, Techno India University, EM-4 Sector-V, Salt Lake City, Kolkata 700091, West Bengal, India. Email: ganguli.arnab009@gmail.com (A. G.) and sandhimita@gmail.com (S. M.) Contribution: ResourcesSearch for more papers by this authorArnab Ganguli, Corresponding Author Arnab Ganguli ganguli.arnab009@gmail.com orcid.org/0000-0001-9939-027X Department of Microbiology, Techno India University, Kolkata, India Correspondence Arnab Ganguli and Sandhimita Mondal, Department of Microbiology, Techno India University, EM-4 Sector-V, Salt Lake City, Kolkata 700091, West Bengal, India. Email: ganguli.arnab009@gmail.com (A. G.) and sandhimita@gmail.com (S. M.) Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draftSearch for more papers by this author Shatabdi Das, Shatabdi Das Department of Microbiology, Techno India University, Kolkata, India Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Project administration, Validation, Visualization, Writing - original draftSearch for more papers by this authorKumari Vishakha, Kumari Vishakha Department of Microbiology, Techno India University, Kolkata, India Contribution: Data curation, Formal analysis, ​Investigation, MethodologySearch for more papers by this authorSatarupa Banerjee, Satarupa Banerjee Department of Microbiology, Techno India University, Kolkata, India Contribution: MethodologySearch for more papers by this authorSandhimita Mondal, Corresponding Author Sandhimita Mondal sandhimita@gmail.com Department of Microbiology, Techno India University, Kolkata, India Correspondence Arnab Ganguli and Sandhimita Mondal, Department of Microbiology, Techno India University, EM-4 Sector-V, Salt Lake City, Kolkata 700091, West Bengal, India. Email: ganguli.arnab009@gmail.com (A. G.) and sandhimita@gmail.com (S. M.) Contribution: ResourcesSearch for more papers by this authorArnab Ganguli, Corresponding Author Arnab Ganguli ganguli.arnab009@gmail.com orcid.org/0000-0001-9939-027X Department of Microbiology, Techno India University, Kolkata, India Correspondence Arnab Ganguli and Sandhimita Mondal, Department of Microbiology, Techno India University, EM-4 Sector-V, Salt Lake City, Kolkata 700091, West Bengal, India. Email: ganguli.arnab009@gmail.com (A. G.) and sandhimita@gmail.com (S. M.) Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draftSearch for more papers by this author First published: 19 May 2021 https://doi.org/10.1111/jfpp.15668Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Pathogenic microorganisms are responsible for spoilage and foodborne illness due to improper post-process treatment. The purpose of this study was to formulate a film that used alginate-vanillin (ALG-VAN) in combination to control food pathogens and extend the shelf life of leafy vegetable lettuce. Initially, we determined minimum inhibitory concentration (MIC) of ALG-VAN in combination against five different foodborne pathogens. This MIC of ALG-VAN also reduced hydrophobicity, autoaggregation, and effectively eradicated sessile cells of biofilm-associated foodborne pathogens. This is an interesting possibility that this combination has been capable of eradicating food pathogens. Hence, we were able to develop a preservative film with acceptable physical properties using this MIC. When used as lettuce packaging material, this film effectively prevents weight loss and reduces bacterial loads compared to commercial LDPE film. Hence, ALG-VAN film may be considered an excellent preservative option for controlling the spoilage and contamination of food-pathogenic bacteria. Novelty impact statement Alginate and vanillin in combination eradicated both sessile and planktonic forms of foodborne pathogens under both mono and dual-species culture conditions. The ALG-VAN film was successfully prepared and characterized, and it was observed to have antibacterial activity against food-borne pathogens. ALG-VAN film delayed the bacterial spoilage and extended the shelf life of lettuce. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available on request from the corresponding author. Volume45, Issue9September 2021e15668 RelatedInformation