软骨
材料科学
细胞外基质
软骨发生
组织工程
去细胞化
生物医学工程
脚手架
阿格里坎
生物材料
再生(生物学)
基质(化学分析)
II型胶原
软骨细胞
自愈水凝胶
生物相容性
生物加工
糖胺聚糖
再生医学
纳米技术
体内
透明软骨
关节软骨
解剖
细胞生物学
骨关节炎
生物
复合材料
病理
医学
替代医学
作者
Maryam Khajavi,Abdolmajid Hajimoradloo,Mojgan Zandi,Mohamad Pezeshki‐Modaress,Shahin Bonakdar,Abbas Zamani
摘要
Here, engineered cartilage-like scaffold using an extracellular matrix (ECM) from sturgeon fish cartilage provided a chondroinductive environment to stimulate cartilaginous matrix synthesis in human adipose stem cells (hASCs). Three dimensional porous and degradable fish cartilage ECM-derived scaffold (FCS) was produced using a protocol containing chemical decellularization, enzymatic solubilization, freeze-drying and EDC-crosslinking treatments and the effect of different ECM concentrations (10, 20, 30, and 40 mg/ml) on prepared scaffolds was investigated through physical, mechanical and biological analysis. The histological and scanning electron microscopy analysis revealed the elimination of the cell fragments and a 3-D interconnected porous structure, respectively. Cell viability assay displayed no cytotoxic effects. The prepared porous constructs of fish cartilage ECM were seeded with hASCs for 21 days and compared to collagen (Col) and collagen-10% hyaluronic acid (Col-HA) scaffolds. Cell culture results evidenced that the fabricated scaffolds could provide a proper 3-D structure to support the adhesion, proliferation and chondrogenic differentiation of hASCs considering the synthesis of specific proteins of cartilage, collagen type II (Col II) and aggrecan (ACAN). Based on the results of the present study, it can be concluded that the porous scaffold derived from fish cartilage ECM possesses an excellent potential for cartilage tissue engineering.
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