Adduct of Aquacobalamin with Hydrogen Peroxide

Aquacobalamin binds hydrogen peroxide reversibly to form a cobalt(III) hydroperoxo adduct with a 0.25 mM dissociation constant, as evidenced by UV–vis absorption spectroscopy and corroborated by NMR, Raman spectroscopy, stopped-flow UV–vis measurements, and density functional theory calculations.