Smartphone-assisted ratiometric fluorescence sensing platform and logical device based on polydopamine nanoparticles and carbonized polymer dots for visual and point-of-care testing of glutathione

As a crucial biothiol, glutathione (GSH) plays a key role in the organisms. Monitoring GSH level is of great significance for disease diagnosis and biomedical research. In this work, polydopamine (PDA) nanoparticles-red fluorescent carbonized polymer dots (r-CPDs) based ratiometric fluorescence sensing platform was constructed and employed for GSH assay. Dopamine (DA) could be oxidized by cobalt oxyhydroxide (CoOOH) nanosheets and further polymerized into PDA nanoparticles with green fluorescence. However, in the presence of GSH, CoOOH nanosheets were reduced and decomposed, which prevented the production of PDA nanoparticles. In the sensing system, green-emitting PDA nanoparticles were employed as a response unit and r-CPDs were used as an internal reference unit. With the addition of GSH, the green fluorescence of PDA nanoparticles decreased as well as the red fluorescence of system remained relatively stable. Importantly, a distinct fluorescence color evolution from green to red was presented with a serious of GSH concentrations. Based on this, a portable smartphone-assisted ratiometric chromaticity analytical method was developed to achieve the on-site visual detection of GSH. Both the established ratiometric fluorescence and ratiometric chromaticity sensing methods for GSH assay have the merits of wide linear range, high sensitivity and excellent accuracy, which are suitable for the determination of GSH in human serum and exhibit great application potential in rapid and accurate monitoring of the GSH levels in clinical. Moreover, an ingenious logical device reflecting GSH levels was designed based on the two different fluorescence signals, which provided a new strategy for the intelligent online detection of GSH in complex biological matrices.