Fabrication of ω‐Transaminase@Metal‐Organic Framework Biocomposites for Efficiently Synthesizing Benzylamines and Pyridylmethylamines

Abstract In this study, ten ω‐transaminases (ω‐TAs) have been investigated to efficiently catalyze the synthesis of twenty‐four functionalized benzylamines and pyridylmethylamines. We optimized the reactions, screened suitable amino donors and compared ω‐transaminases activities for all aromatic aldehyde substrates. Under the optimized conditions, eighteen aromatic amines have been obtained with 60.4%–96.6% conversions and isolated only via simple extraction and recrystallization with 18.5%–81% yields on a preparative scale. Furthermore, we first immobilized the Bm ‐STA onto the MOFs via the physical adsorption to overcome the limitation of free enzyme and improve their industrial applications. The obtained Bm ‐STA/UiO‐66‐NH 2 composites exhibited not only high enzymes loading (80.4 mg g −1 ) and enzyme activity recovery (95.8%), but also the better reusability, storage stability, pH stability and the tolerance to acetone and DMF. magnified image