Structures bounded by directly-oriented members of the IS26 family are pseudo-compound transposons.

转座酶 转座因子 换位(逻辑) 同源重组 生物 重组 遗传学 位点特异性重组 Tn10 DNA 基因 数学 突变体 重组酶 几何学
作者
Christopher J. Harmer,Carol H. Pong,Ruth M. Hall
出处
期刊:Plasmid [Elsevier BV]
卷期号:111: 102530-102530 被引量:40
标识
DOI:10.1016/j.plasmid.2020.102530
摘要

Antibiotic resistance genes are often found in structures bounded by copies of IS 26 , IS 257 /IS 431 or IS 1216 that resemble compound (or composite) transposons. However, because of the mechanisms used by IS 26 family members, namely that they form cointegrates but cannot resolve them, none of these structures can move together as a coherent single unit. Apparent transposition of these structures is possible via a 2-step process but only if the IS are in direct orientation. An intermolecular reaction catalysed by the IS-encoded transposase and an intramolecular homologous recombination step can occur in either order. In one route, one of the IS bounding the structure forms a cointegrate between the DNA molecule carrying it and a target molecule. Cointegrates formed by either copy-in or targeted conservative routes contain three directly-oriented IS copies and can be resolved by homologous recombination between specific pairs of IS, with one pair leading to apparent transposition of the whole structure. In the other route, homologous recombination first forms a circular intermediate, a translocatable unit or TU, which is incorporated by the transposase either at a random site or adjacent to another IS copy in a target molecule. We therefore conclude that the transposon-like structures are not compound (or composite) transposons and the nomenclature for them should be revised. We propose that the term “pseudo compound transposon” (PCT), first coined in 1989, should be used to describe those structures where the IS are in direct orientation. Structures with the IS in opposite orientation should not be named as transposons. • IS 26 family members IS 26 , IS 257 /IS 431 or IS 1216 frequently flank antibiotic resistance genes. • Structures bounded by IS 26 family members resemble compound or composite transposons but cannot move together as a unit • Because of the mechanisms used by IS 26 family members, these structures can only move if the IS are in direct orientation. • Apparent transposition is a 2-step process, usually involving a transposase-catalysed step and homologous recombination. • The term “pseudo compound transposon” (PCT) coined in 1989 better describes these structures.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
酷波er应助hello采纳,获得10
2秒前
2秒前
啦啦发布了新的文献求助10
2秒前
唯美发布了新的文献求助10
6秒前
hnlgdx发布了新的文献求助10
10秒前
一根完成签到,获得积分20
10秒前
七月完成签到 ,获得积分10
12秒前
xiaojiahuo完成签到,获得积分10
13秒前
14秒前
猪猪hero发布了新的文献求助10
14秒前
自信不愁完成签到,获得积分10
15秒前
潇湘雪月发布了新的文献求助10
16秒前
Arthur完成签到,获得积分10
17秒前
17秒前
量子星尘发布了新的文献求助10
18秒前
芋孟齐完成签到,获得积分20
18秒前
猪猪hero发布了新的文献求助10
20秒前
21秒前
高手发布了新的文献求助10
21秒前
21秒前
WN发布了新的文献求助10
21秒前
超级的鹅发布了新的文献求助10
24秒前
FashionBoy应助axin采纳,获得10
25秒前
胡霖完成签到,获得积分10
26秒前
流飒完成签到,获得积分10
26秒前
香蕉觅云应助牛马码字员采纳,获得10
27秒前
猪猪hero发布了新的文献求助10
27秒前
CAOHOU应助林sir采纳,获得10
27秒前
27秒前
甜甜亦巧完成签到,获得积分10
28秒前
栗惠发布了新的文献求助20
29秒前
29秒前
30秒前
32秒前
小二郎应助高手采纳,获得10
32秒前
33秒前
民科王聪发布了新的文献求助10
33秒前
33秒前
YaoZhang完成签到 ,获得积分10
34秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989242
求助须知:如何正确求助?哪些是违规求助? 3531393
关于积分的说明 11253753
捐赠科研通 3270010
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136