Systemic lupus erythematosus: PKCA is an inhibition pathway for mTOR by the active ingredient of green tea

Abstract This study aims to evaluate the interaction of green tea active compounds with proteins related to mTOR signals. The in silico study uses SEA protein target software, DB strings, and AUTODOCK PYRX 9.5. There are twenty target proteins that can interact with the active compounds of green tea. Of the twenty proteins, only six proteins are connected to the mTOR pathway. Of the six proteins, one that is a regulator of mTOR inhibitors is PKCA. Epigallocatechin has the strongest interaction with PKCA 4ARA (-8 kcal / mol). Cianidanol has the strongest interaction with PKCA 3IW4 (-9.3 kcal / mol). To analyze the involvement of the autophagy, a docking between ULK1 and AMPK was conducted, and there was an interaction between ULK1 and AMPK (bond energy of -1446.11 kcal). For the interaction between mTOR and ULK1, the bond energy is -624.5 kcal. For active green tea compounds, the bonding energy is more positive than the mTOR bond with ULK1. It was concluded that the green tea active ingredient as an inhibitor control against mTOR through PKCA and ULK1-AMPK (autophagy pathway).