化学
原人参二醇
色谱法
芍药苷
质谱法
甲酸
人参皂甙
油酸烷
人参
液相色谱-质谱法
梯度洗脱
糖苷
根(腹足类)
三萜
高效液相色谱法
立体化学
替代医学
病理
生物
医学
植物
作者
Ruoliu Wang,Hai‐Qiang Wang,Xiaohui Fan,Mei-Xian Duan,Hongyan Li,Shufang Wang
出处
期刊:PubMed
日期:2020-02-01
卷期号:45 (3): 555-564
被引量:5
标识
DOI:10.19540/j.cnki.cjcmm.20191002.308
摘要
The chemical constituents in Shenmai Injection(SMI) were qualitatively analyzed by using liquid chromatography/quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS) and liquid chromatography-ion trap-mass spectrometry(LC-IT-MS). The analysis was performed on an Agilent Zorbax SB-C_(18)(4.6 mm×250 mm, 5 μm) and gradient elution was carried out with 0.05% formic acid solution-acetonitrile as mobile phase at a flow rate of 0.6 mL·min~(-1) and a column temperature of 30 ℃. Mass spectrometry data of the components in SMI were collected in negative ion mode. The structures of components were speculated and identified by analyzing mass spectrometry data, comparing with standards, and referring to related literature. A total of 64 components in SMI were estimated, and the structures were confirmed in 16 of them by comparison with standards. Fifty-six compounds derived from Ginseng Radix et Rhizoma Rubra included 34 protopanaxadiol ginsenosides, 19 protopanaxatriol ginsenosides, 1 oleanane ginsenosides and 2 other glycosides. Eight compounds derived from Ophiopogonis Radix included 7 steroidal saponins, and 1 monoterpene glycoside. The results of this study would provide an important theoretical basis for the improvement of the quality control standards and the discovery of effective constituents in SMI.
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