米非司酮
右美托咪定
敌手
原肌球蛋白受体激酶B
医学
海马体
麻醉
心理学
内科学
内分泌学
受体
神经营养因子
生物
遗传学
镇静
怀孕
作者
Qing Hua,Chunhui Li,Cheng Tan,Dutian Wang
标识
DOI:10.3760/cma.j.issn.1674-6554.2017.03.003
摘要
Objective
To investigate the effect and mechanism of dexmedetomidine, mifepristone and dexmedetomidine plus mifepristone on the fear memory in rats with post-traumatic stress disorder (PTSD).
Methods
40 male SD rats were randomly divided into five groups with 8 in each group: control group (group C), PTSD model group (group P), dexmedetomidine group (group D), mifepristone group (group M) and dexmedetomidine plus mifepristone group (group U). Fear memory in rats was evaluated by fear conditioning test (FC). Anxiety-like behavior was assessed by the elevated plus-maze test (EPM). Expressions of BDNF and its receptor TrkB in the hippocampus of rats after fear condition were detected using Western blot (WB) and CORT level in the serum was detected using enzyme-linked immunosorbent assay (ELISA).
Results
Compared with group P, the freezing scores in the FC in group D((32.29±8.09) %), M((33.33±8.21) %), and U((9.38±3.31) %) were significantly decreased (P<0.05). The times and entries in the open arms of the EPM were significantly increased (P<0.05). The expressions of BDNF in group D(0.65±0.04), M(0.71±0.04), U(0.79±0.07) and TrkB in group D(0.66±0.04), M(0.71±0.04), U (0.86±0.03) were obviously rescued in hippocampus of rats (P<0.05). The CORT level in serum in group D((37.65±12.37)μg/L) and U((59.10±5.23)μg/L) was decreased (P<0.05). There was no difference between group P and M.
Conclusion
These results suggest that dexmedetomidine, mifepristone and dexmedetomidine plus mifepristone can significantly enhance fear extinction and improve anxiety-like behaviors in rats with PTSD. The mechanism may be that dexmedetomidine and mifepristone could enhance the expressions of BDNF and TrkB in the hippocampus.
Key words:
Dexmedetomidine; Mifepristone; Post-traumatic stress disorder; BDNF; Fear memory extinction
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